K. Ingkaninan et al., Comparison of some centrifugal partition chromatography systems for a general separation of plant extracts, J LIQ CHR R, 22(6), 1999, pp. 885-896
In order to find a Centrifugal Partition Chromatography (CPC) solvent syste
m that can be used as a general prefractionation step for crude plant extra
cts, twelve two-phase systems were compared. The butanol: ethyl acetate: wa
ter 3:2:5 (v/v/v) system gave good separation for polar compounds, and the
heptane: ethyl acetate: methanol: water 6:1:6:1 (v/v/v/v) system was found
to be effective for the separation of nonpolar compounds and somewhat less
for polar compounds.