Ferulic acid, derived from sugar beet pulp, was used as precursor in a biot
echnological two-step process to produce 'natural' vanillin. This process c
ombined the biotransformation of sugar beet pulp ferulic acid to vanillic a
cid by a micromycete, Aspergillus niger and the biotransformation of recove
red vanillic acid into vanillin by a basidiomycete, Pycnoporus cinnabarinus
. The system produced more than 100 mg litre(-1) natural vanillin. The sens
orial analysis of this new natural vanillin revealed, besides a predominant
vanillin flavour, a slight odour of chocolate as a secondary organoleptic
sensation. (C) 1999 Society of Chemical Industry.