Charge derivatization of peptides for analysis by mass spectrometry

The analysis of peptide derivatives by fast atom bombardment, liquid second ary-ionization mass spectrometry, plasma desorption, electrospray ionizatio n, and matrix-assisted laser desorption/ionization is reviewed. The fragmen tation patterns of peptides and of charge-derivatized peptides are compared and the proposed fragment ion structures are summarized. A variety of deri vatization approaches and the distinguishing features of mass spectra produ ced from these derivatives are described. The most promising derivatization approaches are evaluated, and the strengths and limitations of these appro aches are discussed. (C) 1999 John Wiley & Sons, Inc.