Analysis of DNA in single pollen cell by Hadamard transform microscopic fluorescence imaging

Authors
Tang, HW Chen, GQ Zeng, YN
Citation
Hw. Tang et al., Analysis of DNA in single pollen cell by Hadamard transform microscopic fluorescence imaging, MICROCHEM J, 61(3), 1999, pp. 177-182
Citations number
15
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
MICROCHEMICAL JOURNAL
ISSN journal
0026265X → ACNP
Volume
61
Issue
3
Year of publication
1999
Pages
177 - 182
Database
ISI
SICI code
0026-265X(199903)61:3<177:AODISP>2.0.ZU;2-G
Abstract
A noncommercial Hadamard transform microscopic image analysis system was us ed to measure fluorescence emitted by single cells. A method for subtractin g the native fluorescence of the Zephyranthes candida(Lindl.) Herb. pollen cell was developed based on its correlation to pollen area. Acridine orange and ethidium bromide were used as fluorescence probes for pollen DNA quant itative analysis, and the results obtained by the two methods were compared . (C) 1999 Academic Press.