Independent regulation of the two Pax5 alleles during B-cell development

Citation
Sl. Nutt et al., Independent regulation of the two Pax5 alleles during B-cell development, NAT GENET, 21(4), 1999, pp. 390-395
Citations number
30
Categorie Soggetti
Molecular Biology & Genetics
Journal title
NATURE GENETICS
ISSN journal
10614036 → ACNP
Volume
21
Issue
4
Year of publication
1999
Pages
390 - 395
Database
ISI
SICI code
1061-4036(199904)21:4<390:IROTTP>2.0.ZU;2-4
Abstract
The developmental control genes of the Pax family are frequently associated with mouse mutants and human disease syndromes(1-3). The function of these transcription factors is sensitive to gene dosage, as mutation of one alle le(1-3) or a modest increase in gene number(4) results in phenotypic abnorm alities. Pax5 has an important role in B-cell and midbrain development(5-7) . By following the expression of individual Pax5 alleles at the single-cell level, we demonstrate here that Pax5 is subject to allele-specific regulat ion during B-lymphopoiesis. Pax5 is predominantly transcribed from only one allele in early progenitors and mature B cells, whereas it switches to a b iallelic transcription mode in immature B cells. The allele-specific regula tion of Pax5 is stochastic, reversible, independent of parental origin and correlates with synchronous replication, in contrast with imprinted(8,9) an d other monoallelically expressed genes(10,11). As a consequence, B-lymphoi d tissues are mosaics with respect to the transcribed Pax5 allele, and thus mutation of one allele in heterozygous mice results in deletion of the cel l population expressing the mutant allele due to loss of Pax5 function at t he single-cell level. Similar allele-specific regulation may be a common me chanism causing the haploinsufficiency and frequent association of other Pa x genes with human disease.