Expression of mRNA encoding alpha 1E and alpha 1G subunit in the brain of a rat model of absence epilepsy

Low voltage-activated calcium channels are thought to play a key role in th e generation of spike and waves discharges characteristic of absence epilep sy. Therefore, the expression level of mRNA encoding calcium channel alpha 1E and alpha 1G subunits was measured in the brain of genetic absence epile psy rats from Strasbourg (GAERS). Using quantitative RT-PCR and in situ hyb ridization, no difference was found in alpha 1G mRNA expression between GAE RS and control animals, while a decreased expression of alpha 1E was seen i n the cerebellum and the brain stem of the GAERS. This phenomenon was not o bserved in young animals when the epileptic phenotype is not expressed. (C) 1999 Lippincott Williams & Wilkins.