Experimental transmission of Crimean-Congo haemorrhagic fever virus: Role of three vectorial species in maintenance and transmission cycles in Senegal

In this article, we published the role of three species ei ticks Amblyomma variegatum (Fabricius, 1974), Hyalomma marginatum rufipes (Koch, 1844) and Hyalomma truncatum (Koch, 1844) in the maintenance and transmission of the CCHF virus. The images of these species were infected by intracoelomic rout e. Vertical transmission (transtasial and transovarial) and horizontal tran smission for different stases were studied by isolation on newborn mice, po lymerase chain reaction, indirect immunofluorescence and ELISA. Our results proved that 15 days after inoculation, infection rates of 100 % were noted with Hyalomma marginatum rufipes and Hyalomma truncatum. This r ate is about 60 % for Ambiyommo variegatum. The images of the three species infected have transmitted the virus to their host during blood feeding (10 0 %). A high transovarial transmission for Hyalomma marginatum rufipes and Hyalomma truncatum were observed (respectively 53 and 50 %). This rate is a bout 12 % for Amblyomma variegatum. The tick infection does not persist up to the first generation for the three species studied. Ticks ore temporary reservoirs vectors but not permanent reservoirs of CCHF virus.