BIOSYNTHESIS OF VITAMIN-B-12 - USE OF SPECIFIC C-13-LABELING FOR STRUCTURAL STUDIES ON FACTOR IV

Authors
ALANINE AID ICHINOSE K THIBAUT D DEBUSSCHE L STAMFORD NPJ LEEPER FJ BLANCHE F BATTERSBY AR
Citation
Aid. Alanine et al., BIOSYNTHESIS OF VITAMIN-B-12 - USE OF SPECIFIC C-13-LABELING FOR STRUCTURAL STUDIES ON FACTOR IV, Journal of the Chemical Society, Chemical Communications, (2), 1994, pp. 193-196
Citations number
12
Categorie Soggetti
Chemistry
Journal title
Journal of the Chemical Society, Chemical Communications → ACNP
ISSN journal
00224936
Issue
2
Year of publication
1994
Pages
193 - 196
Database
ISI
SICI code
0022-4936(1994):2<193:BOV-UO>2.0.ZU;2-M
Abstract
[1,10,20-C-13(3)]Uro'gen III is unambiguously synthesised for enzymic conversion into precorrin-4 isolated after aerial oxidation as two epi mers of Factor IV, the C-13 NMR spectra of which rigorously confirm th e presence of a C-1 acetyl group in precorrin-4; attachment of the fou rth methyl group at C-17 of precorrin-4 is also confirmed by related C -13-labelling experiments.