Separation and identification of organic acids in root exudates of Lupinusluteus by capillary zone electrophoresis

The separation and identification of a mixture of organic acids by capillar y electrophoresis, with direct detection by UV absorption at a wavelength o f 200 nm is reported. A solution containing the following organic acids was studied: oxalic, formic, fumaric, malic, succinic, citric, acetic and lact ic acid. Separation was achieved using a fused-silica capillary filled with phosphate buffer (200 mM; pH 6) containing N-acetyl-N,N,N-trimethylammoniu m bromide (0.5 mM) as cationic surfactant, necessary to modify the electros motic dow, The composition of organic acids in exudates of Lupinas luteus w as determined by this method. Copyright (C) 1999 John Whey & Sons, Ltd.