Nitric oxide cGMP mediates the spasmolytic action of 3,4 '-dihydroxy-5,5 '-dimethoxybibenzyl from Scaphyglottis livida

Five aromatic compounds, 3,4'-dihydroxy-5,5'-dimethoxybibenzyl (1), batatas in 111 (2), coelonin (3), 3,7-dihydroxy2,4-dimethoxyphenanthrene (4), and 3 ,7-dihydroxy-2,4,8-trimethoxyphenanthrene (5) were isolated from the orchid Scaphyglottis livida (Lindley) Schltr. Compounds 1-5 induced a concentrati on-dependent inhibition of the spontaneous contractions of the rat ileum wi th potencies comparable or higher to that of papaverine. The relaxation evo ked by compounds 1 4 was blocked by L-NAME, an inhibitor of nitric oxide sy nthase. It was also demonstrated that 1 increased cyclic GMP content in rat ileum rings. Compound 1-induced elevation of cGMP was inhibited by L-NAME and ODQ, inhibitors of nitric oxide synthase and soluble guanylyl cyclase, respectively. These results indicate that nitric oxide/cGMP formation const itute the signaling pathway in the spasmolytic action of compound 1.