Digestibility of diets based on corn and soybean meal or soybeans treated b
y roasting or extrusion, with or without an enzyme supplementation, was mea
sured by "true" (Sibbald) methods, by analysis of excreta, and by analysis
of ileal digesta. Only analysis of ileal digesta was able to consistently m
easure differences between soybean and enzyme treatments in the digestibili
ty of CP, starch, fat, and ME. The amino acid (AA) digestibility of the die
ts was measured by analysis of the ileal contents. Whereas enzyme supplemen
tation improved overall CP digestibility by 2.9%, this improvement was not
equal for all AA. Of the AA most important for broilers fed corn-soybean di
ets, the digestibilities of Lys, Met, and Arg were not improved or not impr
oved significantly by the enzyme supplementation; however, that of Val was
improved by 2.3% and that of Thr was improved by 3.0%. A performance trial
demonstrated that enzyme supplementation with equal diet formulation improv
ed BW and the feed conversion ratio by 1.9 and 2.2%, respectively. A second
performance trial compared standard diet formulations with formulations us
ing enzyme supplementation and energy levels that were reduced by the amoun
t of improvement provided by the inclusion of enzyme in the first performan
ce trial. No difference was seen between treatments, showing that the impro
vement of nutrient utilization brought about by enzyme supplementation comp
letely compensated for the reduced energy content. Whereas enzyme supplemen
tation should allow a reduction in CP formulation as well, individual AA we
re not improved equally by supplementation and should also be balanced.