Endoglucanase V catalytic core is a single chain protein of 213 amino acids
. The titration of the ionizable groups of the macromolecule has been perfo
rmed by an isothermal calorimetric technique and the pK(1)(0) and Delta H-1
(0) related to the groups have been estimated and no apparent abnormality w
as observed in the titration behavior. These values are in agreement with t
he expected theoretical ones. (C) 1998 Elsevier Science B.V.