Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens

Citation
R. Galletto et al., Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens, THERMOC ACT, 321(1-2), 1998, pp. 17-21
Citations number
25
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
THERMOCHIMICA ACTA
ISSN journal
00406031 → ACNP
Volume
321
Issue
1-2
Year of publication
1998
Pages
17 - 21
Database
ISI
SICI code
0040-6031(19981102)321:1-2<17:HOPOEV>2.0.ZU;2-3
Abstract
Endoglucanase V catalytic core is a single chain protein of 213 amino acids . The titration of the ionizable groups of the macromolecule has been perfo rmed by an isothermal calorimetric technique and the pK(1)(0) and Delta H-1 (0) related to the groups have been estimated and no apparent abnormality w as observed in the titration behavior. These values are in agreement with t he expected theoretical ones. (C) 1998 Elsevier Science B.V.