Electron tomography of molecules and cells

Electron tomography is the most winery applicable method for obtaining thre e-dimensional information by electron microscopy. It is, in fact, the only method suitable for investigating pleomorphic structures, such as many supr amolecular assemblies, organelles and cells. With the recent development of automated low-dose data-acquisition schemes, it is now possible to study m olecules and cells embedded in vitreous ice. This opens up new horizons for investigating the functional organization of cellular components with mini mal perturbation of the cellular context.