Citation
P. Chrenek et al., Detection of human protein C gene integration in transgenic rabbits by polymerase chain reaction, VET MED-CZ, 44(3), 1999, pp. 79-82
Citations number
15
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
VETERINARNI MEDICINA
ISSN journal
03758427
→ ACNP
Volume
44
Issue
3
Year of publication
1999
Pages
79 - 82
Database
ISI
SICI code
0375-8427(199903)44:3<79:DOHPCG>2.0.ZU;2-I
Abstract
From a total of 200 rabbit pronuclear stage zygotes microinjected with WAP-
hPC gene (mouse whey acid protein promoter - human protein C) were obtained
21 liveborn descendants after transfer to recipients. The integration of t
he WAP-hPC gene in the genome of born rabbits was determined in samples of
DNA, isolated from tissue ol blood by PCR method. The integration was confi
rmed in 2% (4/200), or in 19% (4/21) of F-0 rabbits, in 11% (5/46) of F-1 a
nd in 31% (4/13) of F-2 generation using two primer sets.