CURRENT STRATEGIES FOR PREDICTION OF RETENTION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The desire to model chromatographic retention derives from both theore tical and practical needs. There are fundamental questions concerning the interactions of molecules in separation systems and the modeling o f chromatographic retentions is critical for methods development: This paper reviews the current strategies for predicting retention in HPLC with emphasis on RP-HPLC. Three main categories are discussed: method s utilizing a physical model, methods which assume no model, and metho ds with an abstract or hidden model. The theoretical background, pract ical application, and relative merits of each approach are discussed.