C. Oddoze et al., RAPID AND SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NICOTINE AND COTININE IN NONSMOKER HUMAN AND RAT URINES, Journal of chromatography B. Biomedical sciences and applications, 708(1-2), 1998, pp. 95-101
Citations number
25
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
A simple reversed-phase high-performance liquid chromatographic method
with paired-ion and UV detection has been developed for the rapid qua
ntification of urinary nicotine and cotinine. A one-step solid-liquid
extraction on Exvelut(R) was used. Separation from endogenous substanc
es was achieved with a decreasing flow-rate. With 20 ml of urine for e
xtraction, the limit of quantification was 0.5 ng/ml for cotinine and
5 ng/ml for nicotine; linearity was obtained from 50 to 5000 ng/ml. Th
e intra-and inter-day coefficients of variation were less than 9% for
cotinine and 30% for nicotine. Average recoveries for cotinine were 92
-100% and 47-86% for nicotine. The present method was applied to the u
rine analysis of smokers, nonsmoker children, and experimental animals
. (C) 1998 Elsevier Science B.V.