HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF BOBEL-24 IN BIOLOGICAL SAMPLES FOR PHARMACOKINETIC, METABOLIC AND TISSUE DISTRIBUTION STUDIES

A rapid and simple HPLC method is described for the determination of B obel-24 (2,4,6-triiodophenol) and other iodinated derivatives in biolo gical samples. The sample preparation was liquid-liquid extraction bef ore injection onto the HPLC system. 2,6-Diiodo-4-methylphenol was used as internal standard. Separation was obtained using a reversed-phase column under isocratic conditions. The mobile phase consisted of water -acetonitrile (62:38). 2,4,6-Triiodophenol was detected at 277 nm. Thi s method was used for Bobel-24 determination in plasma, urine, synovia l liquid and different tissues. The assay was applied to pharmacokinet ic studies in dog and horse plasma and different dog tissues for tissu e distribution profiles toxicological and metabolic studies. In additi on, this method for biological samples can be applied to non-biologica l samples such as pharmaceutical formulations in stability studies and quality control assays. (C) 1998 Elsevier Science B.V.