DETERMINATION OF FREE CONCENTRATION OF SAMERIDINE IN BLOOD-PLASMA BY ULTRAFILTRATION AND COUPLED-COLUMN LIQUID-CHROMATOGRAPHY

Authors
EKLUND E NORSTENHOOG C ARVIDSSON T
Citation
E. Eklund et al., DETERMINATION OF FREE CONCENTRATION OF SAMERIDINE IN BLOOD-PLASMA BY ULTRAFILTRATION AND COUPLED-COLUMN LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 708(1-2), 1998, pp. 195-200
Citations number
9
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
708
Issue
1-2
Year of publication
1998
Pages
195 - 200
Database
ISI
SICI code
0378-4347(1998)708:1-2<195:DOFCOS>2.0.ZU;2-S
Abstract
Sameridine is a new candidate drug with both local anaesthetic and ana lgesic properties. The free concentration of sameridine in blood plasm a was determined by coupled-column liquid chromatography. Following ad justment of the pH and the temperature of the plasma samples, the free fraction was prepared by ultrafiltration. The coupled-column liquid c hromatographic system consisted of a reversed-phase column, a cation-e xchange extraction column and a cation-exchange analytical column. Sam eridine was detected by UV determination at 205 nm and the system show ed high selectivity. The limit of quantification was 1 nM and the with in-day precision was 4.6% (R.S.D., n=10). (C) 1998 Elsevier Science B. V.