CHROMATOGRAPHIC STUDIES ON THE ISOLATION OF PEROXYDISULFATE OXIDATION-PRODUCTS OF PRIMAQUINE

Eight compounds from peroxydisulphate oxidation of primaquine were fra ctionated on Bio-Gel P-2 column using water as an eluent. A HPLC metho d employing acetonitrile-methanol-1 M perchloric acid-water (30:7:1:95 , v/v) as a mobile phase at 1.0 ml/min on mu Bondapak reversed-phase c olumn and UV detection at 254 nm was developed for the separation and identification of different oxidation products of primaquine. A combin ation of Bio-Gel chromatography with reversed-phase HPLC was found to be the most suitable analytical technique for the semipreparative isol ation of various products formed from the oxidation. Two oxidation pro ducts that were isolated had three or four times higher gametocytocida l activity as compared to primaquine. (C) 1998 Elsevier Science B.V.