DETERMINATION OF CIPROFLOXACIN IN PLASMA AND URINE BY HPLC WITH ULTRAVIOLET DETECTION

Authors
KAMBERI M TSUTSUMI K KOTEGAWA T NAKAMURA K NAKANO S
Citation
M. Kamberi et al., DETERMINATION OF CIPROFLOXACIN IN PLASMA AND URINE BY HPLC WITH ULTRAVIOLET DETECTION, Clinical chemistry, 44(6), 1998, pp. 1251-1255
Citations number
13
Categorie Soggetti
Medical Laboratory Technology
Journal title
Clinical chemistry → ACNP
ISSN journal
00099147
Volume
44
Issue
6
Year of publication
1998
Part
1
Pages
1251 - 1255
Database
ISI
SICI code
0009-9147(1998)44:6<1251:DOCIPA>2.0.ZU;2-5
Abstract
A simple, sensitive isocratic method for the detection and quantificat ion of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma p roteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C-18 reversed-phase column. The quantification limi t was 0.01 mg/L in plasma and 0.5 mg/L in urine. This method was suffi ciently sensitive for pharmacokinetic studies.