LOW PERCENTAGE OF ALUMINOXAMINE AND FERRIOXAMINE IN UREMIC SERUM AFTER DESFERRIOXAMINE ADMINISTRATION

HPLC was used to study the effectiveness of two different desferrioxam ine (DFO) administration strategies (15 mg/kg DFO, 1 h or 44 h before dialysis) on generation of aluminoxamine and ferrioxamine in five hemo dialysis patients. The percentage of ultrafilterable aluminum and iron in these patients was also investigated by electrothermal atomic abso rption spectrometry. The administration of DFO in both schemes increas ed the ultrafilterable serum aluminum concentrations from a mean of 17 .1 +/- 1.6% to a mean of 75.7 +/- 14.1%. However, 1 h after DFO infusi on, only 38.8 +/- 7.7% of the total serum aluminum was bound to DFO; 4 4 h after DFO infusion, only 15.8 +/- 8.0% was bound. Similar results were obtained for ferrioxamine. These results suggest that the ultrafi lterable serum fraction contains aluminum and iron chelated by DFO and by DFO metabolites, which retain similar metal-chelating abilities.