ALTERED EXPRESSION OF CONSTITUTIVE TYPE AND INDUCIBLE TYPE HEAT-SHOCKPROTEINS IN RESPONSE OF D-GALACTOSAMINE-SENSITIZED MICE TO LIPOPOLYSACCHARIDE AS AN EXPERIMENTAL ENDOTOXIC-SHOCK MODEL

The expression of heal shock proteins (HSPs) as stress-induced protein s was studied in mice injected with D-galactosamine (D-GalN) and lipop olysaccharide (LPS) as an experimental endotoxic shock model. The expr ession of constitutive type heat shock protein 70 (HSC70) was signific antly reduced in livers of mice injected with D-galactosamine and lipo polysaccharide, while its expression was unaffected in livers of mice injected with D-galactosamine or lipopolysaccharide alone. The express ion of other constitutive type heat shock proteins, namely HSP60, HSP3 2 and HSP25 was also reduced in mice injected with D-galactosamine and lipopolysaccharide. On the other hand, inducible type HSP70 was detec ted in livers from mice injected with D-galactosamine and lipopolysacc haride, but not in livers from mice injected with D-galactosamine or l ipopolysaccharide alone. Simultaneous injection of anti-tumor necrosis factor (TNF)-alpha antibody prevented the liver from reduced expressi on of constitutive type HSC70, and lead to marked expression of induci ble type HSP70 in the liver. Reduced expression of constitutive type H SC70 was also found when D-galactosamine and recombinant TNF-alpha was injected. Therefore, TNF-alpha was suggested to play a critical role on altered expression of constitutive HSC70 and inducible type HSP70 i n response of D-galactosamine-sensitized mice to lipopolysaccharide. ( C) 1998 Published by Elsevier Science B.V.