CHARACTERIZATION OF DIFFERENTIALLY EXPRESSED GENES IN THE BOVINE-T LYMPHOMA CELL-LINE

A differential display (D.D.) analysis was made to detect differential ly expressed genes in a bovine T lymphoma cell line, BTL-26, derived f rom the calf type of sporadic bovine lymphosarcoma. A D.D. analysis co mparing BTL-26 with the bovine epithelial cell line CKT-1 and healthy bovine thymocytes yielded 24 cDNA clones. The DNA sequencing analysis followed by a homology search showed that 20 of the 24 cDNA clones had no significant homology to any sequences in DNA data base. The remain ing four genes were homologous to known sequences. Northern blot hybri dization among BTL-26, CKT-1 and healthy bovine thymocytes showed that a cDNA clone, BC8, was differentially expressed in BTL-26. The clonin g of full-length cDNA for the BC8 clone and its DNA sequences showed t hat the BC8 clone is a bovine nuclear domain protein homologous to the human NDP52 gene. Northern blot analysis showed that the BC8 clone bo vine NDP52 was predominantly expressed in tumor cell line BTL-26, comp ared with the transcripts from several bovine tissues. (C) 1998 Elsevi er Science B.V.