METABOLIC CONVERSION OF 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHANE (DDD) TO 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHYLENE (DDE) IN THE MALE F344 NCR RAT/

1,1-Dichloro-2,2-bis(p-chlorophenyl)ethane (DDD) and 1,1-dichloro-2,2- bis(p-chlorophenyl)ethylene (DDE) levels were measured by capillary ga s chromatography with electron capture detection in liver and blood se rum of male F344/NCr rats exposed for 2 weeks to DDD at dietary concen trations ranging from 8.51 ppm to 2,000 ppm. DDD burdens in serum rang ed from <0.006 mu M (limit of detection) in control rats to 1.1 mu M i n the rats fed DDD at 2,000 ppm. The corresponding liver burdens in th ese animals ranged from <0.006 mu mol/kg liver (controls) to 11 mu mol /kg liver in rats fed DDD at 2,000 ppm. Levels of DDE in serum or live r were undetectable (<0.006 mu M in serum; <0.006 mu mol/kg liver) in rats fed control diet or diet containing 8.51 or 25.5 ppm DDD. The liv er and serum burdens of DDE increased with dietary DDD concentration, reaching a maximum of 0.53 mu M in serum and 4.7 mu mol/kg liver in ra ts fed 2,000 ppm DDD. As a percentage of total DDD equivalents detecte d in liver or serum, the DDE burdens increased to a maximum of 36% and 31% in the serum and liver, respectively, of rats fed 689 ppm DDD. Th e possibility that the DDE might have been generated artifactually in the diet prior to administration to the rats was ruled out by analysis with capillary gas chromatography of the diet containing 2,000 ppm DD D, The identification of DDE as a metabolite in liver extracts of rats fed 2,000 ppm DDD was confirmed with GC-MS. The results confirmed the presence of DDE as a metabolite of DDD.