HIGH-FREQUENCY ADVENTITIOUS SHOOT REGENERATION IN SAINFOIN

A procedure is described for the rapid and efficient adventitious shoo t regeneration from leaflets, petioles and stems of field-grown sainfo in plants. All explants formed shoots on a range of media supplemented with 6-benzyladenine (BA) and alpha-naphthaleneacetic acid (NAA). Ste m explants appeared to have better regeneration capacity than leaflet and petiole explants in most media tested. The highest frequency of sh oot regeneration was achieved from stem segments on a medium containin g 20 mu M BA and 0.5 mu M NAA. Regenerated shoots rooted in half-stren gth Murashige and Skoog medium containing 5 mu M indole-3-butyric acid and later established well under greenhouse conditions.