Hv. Melendez et al., A VALIDATED TECHNIQUE FOR THE ANALYSIS OF BILIARY BILE-ACID SECRETIONIN DONOR LIVERS PRIOR TO TRANSPLANTATION, Transplant international, 11(3), 1998, pp. 216-222
Many parameters currently used for the pre-transplant assessment of li
ver allografts, are not reliable enough in predicting the likelihood o
f early graft dysfunction or non-function. it is generally accepted th
at bile secretion is a sign of hepatic function post-transplant and th
at bile flow shows a close linear relationship to the secretion of bil
e acids (''apparent choleretic activity''). We have studied bile flow,
biliary bile acid concentrations and composition and measured apparen
t choleretic activity from hepatic bile collected with a new technique
under controlled conditions at the time of retrieval from 18 donor li
vers. More than three samples were collected from each of 13 donors an
d a total of 65 samples of hepatic bile were analysed. Of these, ten s
howed typical apparent choleretic activity with a positive slope in th
e regression line analysis (correlation coefficient of 0.9), validatin
g our collection technique.