INTERRESIDUE QUIET NOES FOR DNA STRUCTURAL STUDIES

The potential utility of long-range NOEs in DNA has not been exploited since the observed signals have contributions both from the direct ma gnetization route and from multiple diffusion pathways. The Quiet NOE approach can be used to select for the direct magnetization transfer p athway by suppressing spin diffusion. A single-band Quiet NOE, which a llows detection of the direct NOEs between protons in a selected chemi cal shift window, has been demonstrated on two duplex DNAs, and the NO Es observed can contain important structural information. (C) 1998 Aca demic Press.