Jw. Allen et al., SPERMATID MICRONUCLEUS ANALYSES OF TRICHLOROETHYLENE AND CHLORAL HYDRATE EFFECTS IN MICE, MUTATION RESEARCH, 323(1-2), 1994, pp. 81-88
Mice were exposed by inhalation to trichloroethylene (TCE) or by i.p.
injection to the TCE metabolite, chloral hydrate (CH). Early spermatid
s were analyzed for micronucleus (MN) frequency and the presence or ab
sence of kinetochore(s) using fluorochrome-labeled anti-kinetochore an
tibodies. It was determined that 5 consecutive days of exposure to 5,
50 or 500 ppm TCE during preleptotene through early pachytene stages o
f meiotic cell development do not result in increased frequencies of s
permatid MN, CH at 41, 83 or 165 mg/kg was positive for spermatid MN i
nduction when treatments corresponded to spermatogonial stem cell or p
releptotene spermatocyte stages of development; negative results were
obtained after treatments of leptotene-zygotene or diakinesis-metaphas
e stages. The significantly increased levels of MN observed were invar
iably of the kinetochore-negative type.