USE OF GENERIC FAST GRADIENT LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROSCOPY IN QUANTITATIVE BIOANALYSIS

Short narrow analytical HPLC columns have been used successfully with high linear flow-rates and combined with mass spectrometric detection to produce a generic approach to quantitative bioanalysis. The approac h has been used to validate several assays in the low ng/ml region and an example is given in this paper. When combined with a simple solid- phase extraction process the need for complicated, time consuming meth od development has been removed for the majority of pharmaceutical com pounds. The approach takes advantage of not only the extra selectivity of the MS-MS detector but the excellent resolution and peak shape pro duced by gradient elution. (C) 1998 Elsevier Science B.V. All rights r eserved.