DETERMINATION OF INDOMETHACIN RESIDUES IN POULTRY BY HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY

A HPLC method using a C-18 column and UV detection (254 nm) is describ ed for the determination of indomethacin residues in chicken tissues ( liver, muscle and fat). Drug extraction from tissue homogenate in phos phate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). lndomethacin de tection limit was 20 ng/g for the studied tissues. After administratio n of an oral dose of indomethacin (2 mg/kg), only three of the eight p oultry studied showed drug tissue levels, in those cases the levels we re below 50 ng/g, (C) 1998 Elsevier Science B.V. All rights reserved.