EMULATION OF AN ELISA READER AND QUANTITATIVE IMAGE-ANALYSIS USING THE COMPUTER-PROGRAM DIGITAL-OD

Here, we present a Microsoft(R) Windows(R) application that has been w ritten to emulate a microplate reader. After scanning a standard 96-we ll microplate, color gradations in the slots can be analyzed quantitat ively and be used for the estimation of enzyme kinetics, binding assay s or concentration determination. In a test, series accuracy of the pr ogram was determined We show that the returned optical densities corre lated highly with the true concentrations (r >0.97). Thus, the softwar e is useful for a wide number of applications. Further the program is easy to handle and is available free of charge.