S. Jansen et al., EMULATION OF AN ELISA READER AND QUANTITATIVE IMAGE-ANALYSIS USING THE COMPUTER-PROGRAM DIGITAL-OD, BioTechniques, 24(6), 1998, pp. 998-1001
Here, we present a Microsoft(R) Windows(R) application that has been w
ritten to emulate a microplate reader. After scanning a standard 96-we
ll microplate, color gradations in the slots can be analyzed quantitat
ively and be used for the estimation of enzyme kinetics, binding assay
s or concentration determination. In a test, series accuracy of the pr
ogram was determined We show that the returned optical densities corre
lated highly with the true concentrations (r >0.97). Thus, the softwar
e is useful for a wide number of applications. Further the program is
easy to handle and is available free of charge.