EFFECTS OF TEMPERATURE ON GROWTH AND INTRACELLULAR PROTEINS OF NORWEGIAN TYPHULA-ISHIKARIENSIS ISOLATES

Growth of two groups of strains (group I and III) of the snow mould fu ngus, Typhula ishikariensis from northern Norway, were studied in agar and liquid culture. All strains could grow from -5 to 15 (group III) or 20 degrees C (group I) and the optimum temperatures for growth were 4 or 10 degrees C, respectively. The group III strains showed irregul ar growth as the hypal extensions were inhibited at 15 degrees C. Diff erent temperature two-dimensional electrophoresis revealed that in the group III strains some intracellular proteins were modified at the hi ghest temperature. One of the reasons that group III strains did not g row or showed irregular growth at 20 or 15 degrees C, was probably due to protein aggregation or dissociation and thereby loss of vital prop erties of intracellular proteins.