THROMBIN, A REGULATOR OF REPARATION PROCE SSES IN WOUND-HEALING

Thrombin, binding to receptors of the protease activated receptor (PAR ) family, is involved in wound healing by inducing the reparation proc esses and regulating the activity of mast cells, which secrete mediato rs of inflammation. Using thrombin receptor agonist peptide (TRAP-6) f or the activation of rat mast cells, effect of several receptors, incl uding PAR-1, on mast cells was demonstrated. It was shown that TRAP in creases the concentration of Ca2+ in the cytoplasm of mast cells and r egulates cell degranulation, while releasing nitrogen oxide. Thrombin encapsulated in poly(N-vinyl caprolactam)-calcium alginate (PVCL-Ca-Al g) hydrogel films promotes wound healing in rats as demonstrated by th e acceleration of fibroblast proliferation and neovascularization.