RESPONSE OF AQUATIC BACTERIAL-POPULATIONS TO SUBSTRATE ENRICHMENT

The responses of natural bacterial populations in the waters of the Ki el Fjord, Germany, and in Lake Kinneret, Israel, to additions of organ ic substrates were followed by determining changes over 24 h either in direct cell counts or in H-3-thymidine incorporation, and in the Kiel Fjord additionally in H-3-leucine incorporation. In parallel, 1 mu m filtered water samples were stored for 3 or 4 d in order to starve the indigenous bacterial populations prior to repeating the substrate add ition experiments. Generally, upon substrate addition, relatively high er incorporation of radiotracers was noted in the preincubated samples . Growth response to substrate addition even in starved populations wa s only significant after 24 h. Incorporation rates of H-3-thymidine an d H-3-leucine were more sensitive indicators of bacterial response to substrate additions than cell counts. Continued cell replication in un supplemented controls, and insignificant increase over time of radiotr acer incorporation in most of the fresh samples with added supplements , indicated that the indigenous bacterial populations in Kiel Fjord an d Kinneret were apparently not substrate limited. Comparison of actual bacterial production after 24 h (direct counts) to that predicted by H-3-thymidine incorporation after 1 h showed that although reasonably good predictions of daily production were obtained in the unsupplement ed samples, this was usually not the case when substrates were added.