ENZYMATIC-SYNTHESIS, ISOLATION, AND ANALYSIS OF NOVEL ALPHA-GALACTOSYL-CYCLOISOMALTO-OCTAOSES AND BETA-GALACTOSYL-CYCLOISOMALTO-OCTAOSES

Authors
KOIZUMI K TANIMOTO T KUBOTA Y KITAHATA S
Citation
K. Koizumi et al., ENZYMATIC-SYNTHESIS, ISOLATION, AND ANALYSIS OF NOVEL ALPHA-GALACTOSYL-CYCLOISOMALTO-OCTAOSES AND BETA-GALACTOSYL-CYCLOISOMALTO-OCTAOSES, Carbohydrate research, 305(3-4), 1997, pp. 393-400
Citations number
6
Categorie Soggetti
Chemistry Applied","Chemistry Inorganic & Nuclear",Biology
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
305
Issue
3-4
Year of publication
1997
Pages
393 - 400
Database
ISI
SICI code
0008-6215(1997)305:3-4<393:EIAAON>2.0.ZU;2-Z
Abstract
Novel branched cycloisomalto-octaoses (CI(8)s) were enzymatically synt hesized by transgalactosylation galactosylation with alpha-galactosida se from coffee bean and beta-galactosidase preparations from Penicilli um multicolor and Bacillus circulans, using melibiose and lactose as d onor substrates, and CI8 which is a cyclic homogeneous oligosaccharide composed of eight glucose units bound by alpha-(1 --> 6)-linkages, as an acceptor. alpha-Galactosyl-CI(8)s and beta-galactosyl-CI(8)s obtai ned were isolated and purified by HPLC. Their structures were elucidat ed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOFMS) and NMR spectroscopy. (C) 1998 Elsevier Scie nce Ltd.