THE CHEMOENZYMATIC SYNTHESIS OF THE CORE TRISACCHARIDE OF N-LINKED OLIGOSACCHARIDES USING A RECOMBINANT BETA-MANNOSYLTRANSFERASE

Authors
WATT GM REVERS L WEBBERLEY MC WILSON IBH FLITSCH SL
Citation
Gm. Watt et al., THE CHEMOENZYMATIC SYNTHESIS OF THE CORE TRISACCHARIDE OF N-LINKED OLIGOSACCHARIDES USING A RECOMBINANT BETA-MANNOSYLTRANSFERASE, Carbohydrate research, 305(3-4), 1997, pp. 533-541
Citations number
37
Categorie Soggetti
Chemistry Applied","Chemistry Inorganic & Nuclear",Biology
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
305
Issue
3-4
Year of publication
1997
Pages
533 - 541
Database
ISI
SICI code
0008-6215(1997)305:3-4<533:TCSOTC>2.0.ZU;2-M
Abstract
The chemical synthesis of the beta-mannosyl linkage of N-glycans has p resented a great challenge to synthetic carbohydrate chemists. We have therefore investigated the application of beta-mannosyltransferases t o the preparative synthesis N-linked core oligosaccharides. In this pa per we report the chemoenzymatic synthesis of -(1-->4)-2-acetamido-2-d eoxy-alpha-D-glucopyranose on a preparative scale using a phytanyl-lin ked acceptor in the presence of a recombinant beta-(1-->4)-mannosyltra nsferase. (C) 1998 Elsevier Science Ltd.