N. Niimura et al., NEUTRON LAUE DIFFRACTOMETRY WITH AN IMAGING PLATE PROVIDES AN EFFECTIVE DATA-COLLECTION FOR NEUTRON PROTEIN CRYSTALLOGRAPHY, Physica. B, Condensed matter, 241, 1997, pp. 1162-1165
Neutron Laue diffraction 2 Angstrom data from tetragonal hen egg-white
(HEW) lysozyme were collected for 10 days with neutron imaging plates
. All the 960 hydrogen atoms in the lysozyme and 157 bound water molec
ules could be identified. The final R-factor is 19.5%. Several interes
ting features of hydrogen atoms and bound water molecules of HEW-lysoz
yme were found as follows. (1) The crystal was grown at pH 7.0. There
are no hydrogen (deuterium) atoms bound to carboxylate oxygen atoms of
Glu35 and Asp52, which explains why lysozyme is less active at pH 7.0
. (2) Hydrogen atoms and deuterium atoms are clearly identified. As an
example, the omit map around indol ring of Trp 111 is shown. (C) 1998
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