F. Mizutani et al., GLUCOSE-OXIDASE POLYION COMPLEX-BILAYER MEMBRANE FOR ELIMINATION OF ELECTROACTIVE INTERFERENTS IN AMPEROMETRIC GLUCOSE SENSOR, Analytica chimica acta, 364(1-3), 1998, pp. 173-179
An amperometric glucose-sensing electrode was prepared by immobilizing
glucose oxidase (GOx) on a polyion complex membrane. First, a monolay
er of 3-mercaptopropionic acid (MPA) was made on the surface of a gold
electrode by immersing it in an ethanol solution containing MPA. Aque
ous solutions of poly-L-lysine and poly-4-styrenesulfonate were succes
sively placed on the electrode surface and allowed to dry. A GOx layer
was then formed on the poly-L-lysine/poly-4-styrenesulfonate-complex
layer by crosslinking the enzyme by the addition of a glutaraldehyde s
olution. The polyion complex layer was effective for eliminating elect
rochemical interferents such as L-ascorbic acid, uric acid and acetami
nophen. whereas the hydrogen peroxide produced through the GOx-catalyz
ed reaction permeated rapidly through the layer. This resulted in a ra
pid response (100% response in <5 s) with a low interference level (e.
g., the ratio of response for L-ascorbic acid to that for the same con
centration of glucose was 0.07). The electrode was applied to the assa
y of glucose in beverages and sera, and could be used for more than tw
o months, (C) 1998 Elsevier Science :B.V.