POPC giant liposomes were incubated with pullulan that was hydrophobiz
ed by cholesteryl groups and labelled by a fluorescent marker (FITC).
Confocal laser microscopy clearly showed coating of the outer surface
of the Liposomes with the polymer. This is the first and direct observ
ation of the supramolecular assembly of an artificial cell wall onto c
ell-sized liposomes.