MICRODISSECTION AND AMPLIFICATION OF THE CHROMOSOME ARM 5S IN A RICE TELO-TETRASOMIC

The rice aneuploids with telochromosomes are ideal generic stocks for chromosome arm identification and microdissection. A rice telo-tetraso mic line with two extra short arms of chromosome 5 (5S) was used in th e present study. The arms oi 5S were microdissected from the prometaph ase cells in mitosis and amplified with linker adaptor PCR (LA-PCR). T he amplified fragments, ranging from 200-3000 bp, were confirmed to be from the rice chromosome arm 5S by the rice STS and microsatellite ma rkers.