PRODUCTION OF MONOCLONAL-ANTIBODIES TO 6 ISOTYPES OF HORSE IMMUNOGLOBULIN

Stable clones of 18 mouse hybridomas that produce monoclonal antibodie s (MAbs) to six isotypes of horse immunoglobulin were produced. The nu mber of hybridomas. of different specificities are: four to IgGa, four to IgGb, one to IgGc, four to IgG(T), two to IgM, and three to IgA. T he immunoglobulin isotypes purified from affinity columns bound with e ach MAb showed variable mobility in agarose-gel electrophoresis. Migra tion proceeded from the anode as: IgG(T), IgA, IgM, IgGc, IgGb and IgG a. The purified isotypes also demonstrated a single precipitin line wh en in immunoelectrophoresis against isotype-specific or polyclonal ant isera, raised against the purified isotype or whole equine serum, resp ectively. (C) 1998 Elsevier Science B.V.