PHYSICAL ASSIGNMENT OF 2 PORCINE MICROSATELLITES TO THE SHORT ARM OF CHROMOSOME-1 BY MODIFIED DISC-PCR

Direct in situ single copy PCR (DISC-PCR) was modified to increase sig nal detection efficiency and physically assign two porcine microsatell ites (MS), Sw552 and Sw137, to chromosome 1p at Flpter 0.1-0.2 and Flp ter 0.01-0.2, respectively. These are the first two MS localized to ch romosome Ip by an in situ method. The modifications of the DISC PCR pr ocedure included use of buffered formalin and pepsin as pre-reaction t reatments, and tetramethylbenzidine (TMB) for signal detection. The de tection efficiency of the modified method is twofold higher than that of the original technique.