THE MTCY428.08 GENE OF MYCOBACTERIUM-TUBERCULOSIS CODES FOR NAD(+) SYNTHETASE

Authors
CANTONI R BRANZONI M LABO M RIZZI M RICCARDI G
Citation
R. Cantoni et al., THE MTCY428.08 GENE OF MYCOBACTERIUM-TUBERCULOSIS CODES FOR NAD(+) SYNTHETASE, Journal of bacteriology, 180(12), 1998, pp. 3218-3221
Citations number
21
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
180
Issue
12
Year of publication
1998
Pages
3218 - 3221
Database
ISI
SICI code
0021-9193(1998)180:12<3218:TMGOMC>2.0.ZU;2-J
Abstract
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD(+) synthetases. The MTCY428.08 gen e was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expres sion in Escherichia coli led to overproduction of glutathione S-transf erase fused and His(6)-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCP428.08 gene of M. tuberculosis is the structural gene for NAD(+) s ynthetase.