THE ARCANOBACTERIUM (ACTINOMYCES) PYOGENES PLASMID PAP1 IS A MEMBER OF THE PIJ101 PJV1 FAMILY OF ROLLING CIRCLE REPLICATION PLASMIDS/

Authors
BILLINGTON SJ JOST BH SONGER JG
Citation
Sj. Billington et al., THE ARCANOBACTERIUM (ACTINOMYCES) PYOGENES PLASMID PAP1 IS A MEMBER OF THE PIJ101 PJV1 FAMILY OF ROLLING CIRCLE REPLICATION PLASMIDS/, Journal of bacteriology, 180(12), 1998, pp. 3233-3236
Citations number
26
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
180
Issue
12
Year of publication
1998
Pages
3233 - 3236
Database
ISI
SICI code
0021-9193(1998)180:12<3233:TA(PPP>2.0.ZU;2-Q
Abstract
The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes ha d sequence similarity within the putative replication protein and doub le-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermedi ates of pJGS84 replication suggested that this plasmid replicates by t he rolling circle mechanism.