PREVALENCE OF BANDS OTHER THAN 160 AND 130 KDA IN PEMPHIGUS SERA (A MULTICENTER IMMUNOBLOTTING STUDY)

Patients with pemphigus may produce antibodies against molecules other than the classical transmembranal ones. Recently, for example antibod ies to 230 kDa antigens have been found in association with antibodies to intercellular substance. To better understand their prevalence, cl inical correlates and prognostic significance of bands other than 130 and 160 kDa, we studied 67 pemphigus sera. About one-fourth of patient s revealed multiple heterogeneous bands and 13% the 230 kDa band. When challenged with the recombinant protein rBP55, the carbossiterminal p ortion of bullous pemphigoid major antigen, all 230 kDa-positive-sera proved negative. Caution is to be recommended in interpreting pemphigu s sera with a band migrating at the 230 kDa level. (C) 1998 Elsevier S cience B.V.