A METHOD FOR THE POSITIVE SELECTION OF SPONTANEOUS RHIZOBIUM MUTANTS SHOWING TRANSCRIPTIONAL ACTIVATION OF NODULATION GENES IN THE ABSENCE OF NOD-INDUCERS
Jm. Vinardell et al., A METHOD FOR THE POSITIVE SELECTION OF SPONTANEOUS RHIZOBIUM MUTANTS SHOWING TRANSCRIPTIONAL ACTIVATION OF NODULATION GENES IN THE ABSENCE OF NOD-INDUCERS, Molecular plant-microbe interactions, 6(6), 1993, pp. 782-785
A plasmid (pMUS248) containing a promoterless tetracycline resistance
gene under the control of the Rhizobium leguminosarum bv. viciae nodA
gene was constructed. Selection of tetracycline resistance in the abse
nce of naringenin allowed the selection of spontaneous Rhizobium mutan
ts showing transcriptional activation of nodulation genes in the absen
ce of nod-inducers. These mutants were effective on Glycine max.