A METHOD FOR THE POSITIVE SELECTION OF SPONTANEOUS RHIZOBIUM MUTANTS SHOWING TRANSCRIPTIONAL ACTIVATION OF NODULATION GENES IN THE ABSENCE OF NOD-INDUCERS

Citation
Jm. Vinardell et al., A METHOD FOR THE POSITIVE SELECTION OF SPONTANEOUS RHIZOBIUM MUTANTS SHOWING TRANSCRIPTIONAL ACTIVATION OF NODULATION GENES IN THE ABSENCE OF NOD-INDUCERS, Molecular plant-microbe interactions, 6(6), 1993, pp. 782-785
Citations number
16
Categorie Soggetti
Plant Sciences
ISSN journal
08940282
Volume
6
Issue
6
Year of publication
1993
Pages
782 - 785
Database
ISI
SICI code
0894-0282(1993)6:6<782:AMFTPS>2.0.ZU;2-4
Abstract
A plasmid (pMUS248) containing a promoterless tetracycline resistance gene under the control of the Rhizobium leguminosarum bv. viciae nodA gene was constructed. Selection of tetracycline resistance in the abse nce of naringenin allowed the selection of spontaneous Rhizobium mutan ts showing transcriptional activation of nodulation genes in the absen ce of nod-inducers. These mutants were effective on Glycine max.