DISTRIBUTION OF THE RECEPTOR FOR A NOVEL PEPTIDE STIMULATING PHOSPHOINOSITIDE HYDROLYSIS IN HUMAN-LEUKOCYTES

Objectives: Previously we reported that a synthetic peptide, WKYMVm, s timulates phosphoinositide (PI) hydrolysis in several hematopoietic ce ll lines and human neutrophils. In addition, the peptide induces super oxide generation in human neutrophils. The biochemical stimulation app eared to be mediated by specific receptors on leukocytes. In this repo rt we try to find whether the specific receptor(s) exists on specific types of cells in human leukocytes. Design and Methods: To study distr ibution of the peptide-specific receptors, we prepared a probe, biotin -labeled WKYMVm, and analyzed distribution of the peptide receptor usi ng a biochemical study, cytochemical staining, and flow cytometric ana lysis. Results: The peptide-specific receptors are expressed in severa l human leukocytes including granulocytes, monocytes, and B-lymphocyte s but not T-lymphocytes and erythrocytes. Conclusions: These data sugg est that the receptors for the peptide restricted to certain types of leukocytes and this specificity is probably related to the specific fu nctions of the stimulated leukocytes. Copyright (C) 1998 The Canadian Society of Clinical Chemists.