DIRECT IDENTIFICATION OF EACH SPECIFIC MUTATION IN CODON-12 AND CODON-13 OF CI-KI-RAS2 BY SSCP ANALYSIS

We compared the SSCP behaviour of the DNA fragments containing c-ki-ra s 2 wild type 12 and 13 codons or each of the 12 possible point mutate d sequences in these two codons. We found that a single electrophoresi s condition was sufficient to distinguish each specific mutation from the other 11 and from the wild type sequence. This observation makes i t possible to identify each specific mutation directly by SSCP without any need for reamplification and sequencing. (C) 1998 Academic Press.