SIMPLIFIED METHOD FOR VIBRIOCIDAL ANTIBOD Y TITRATION PERFORMED ON A DROP OF BLOOD

We report a simplified technique for vibriocidal antibody test in unde rprivileged people in tropical area. The test is performed on a drop o f blood sampled on a disc of blotting paper. It avoid taking of large quantities of blood in timid underfed people who are often solicited. Its reading is rapid and easy by the naked eye owing to staining viabl e germs violet by the neotetrazolium succinate. Tested in mice and hum ans, the simplified method gave data well correlated to those obtained with the standard test on serum for vibriocidal titres higher than (o r equal to) 1/40.