DETERMINATION OF ARSENIC SPECIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ULTRASONIC NEBULIZATION ATOMIC FLUORESCENCE SPECTROMETRY

A novel technique involving the coupling of high-performance liquid ch romatography with atomic fluorescence spectrometry using ultrasonic ne bulization as an interface was assessed for the determination of As-II I, As-V, dimethylarsinic and monomethylarsonic acid, The effects of hy drogen and argon gas flow rates, nebulization temperature, loop volume , pump rate and phosphate buffer conditions on signal intensities were investigated. The detection limit for As-III, As-V, DMAs and MMAs wer e 35, 50, 20 and 20 ng, respectively (for 250 mm(3) volume injected), Linearity ranges were 250-2500 ng for all four arsenic species.